Susceptibility of human and rat neural cell lines to infection by SARS-coronavirus.
Identifieur interne : 004604 ( Main/Exploration ); précédent : 004603; suivant : 004605Susceptibility of human and rat neural cell lines to infection by SARS-coronavirus.
Auteurs : Makiko Yamashita [Japon] ; Masanobu Yamate ; Gui-Mei Li ; Kazuyoshi IkutaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Humains, Lignée cellulaire, Neurones (anatomopathologie), Neurones (virologie), Rats, Réplication virale (physiologie), Susceptibilité à une maladie (anatomopathologie), Susceptibilité à une maladie (virologie), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- anatomopathologie : Neurones, Susceptibilité à une maladie, Syndrome respiratoire aigu sévère.
- pathogénicité : Virus du SRAS.
- physiologie : Réplication virale, Virus du SRAS.
- virologie : Neurones, Susceptibilité à une maladie, Syndrome respiratoire aigu sévère.
- Animaux, Humains, Lignée cellulaire, Rats.
English descriptors
- KwdEn :
- Animals, Cell Line, Disease Susceptibility (pathology), Disease Susceptibility (virology), Humans, Neurons (pathology), Neurons (virology), Rats, SARS Virus (pathogenicity), SARS Virus (physiology), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology), Virus Replication (physiology).
- MESH :
- pathogenicity : SARS Virus.
- pathology : Disease Susceptibility, Neurons, Severe Acute Respiratory Syndrome.
- physiology : SARS Virus, Virus Replication.
- virology : Disease Susceptibility, Neurons, Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Humans, Rats.
Abstract
Pathological characterization of autopsied tissues from patients with SARS revealed severe damage in restricted tissues, such as lung, with no apparent cell damage in other tissues, such as intestine and brain. Here, we examined the susceptibility of neural cell lines of human (OL) and rat (C6) origins to SARS-associated coronavirus. Both of the neural cell lines showed no apparent cytopathic effects (CPE) by infection but produced virus with infectivity of 10(2-5) per ml, in sharp contrast to the production by infected Vero E6 cells of >10(9) per ml that showed a lytic infection with characteristic rounding CPE. Interestingly, the infection of intestinal cell line CaCo-2 also induced no apparent CPE, with production of the virus at a slightly lower level as that of the Vero E6 cell culture. Notably, the cellular receptor for the virus, angiotensin-converting enzyme 2 was expressed at similar levels on Vero E6 and CaCo-2 cells, but at undetectable levels on OL and C6 cells.
DOI: 10.1016/j.bbrc.2005.06.061
PubMed: 15992768
Affiliations:
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Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name></author><author><name sortKey="Li, Gui Mei" sort="Li, Gui Mei" uniqKey="Li G" first="Gui-Mei" last="Li">Gui-Mei Li</name></author><author><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Disease Susceptibility (pathology)</term><term>Disease Susceptibility (virology)</term><term>Humans</term><term>Neurons (pathology)</term><term>Neurons (virology)</term><term>Rats</term><term>SARS Virus (pathogenicity)</term><term>SARS Virus 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xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Disease Susceptibility</term><term>Neurons</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réplication virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Neurones</term><term>Susceptibilité à une maladie</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Disease Susceptibility</term><term>Neurons</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Rats</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Lignée cellulaire</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pathological characterization of autopsied tissues from patients with SARS revealed severe damage in restricted tissues, such as lung, with no apparent cell damage in other tissues, such as intestine and brain. Here, we examined the susceptibility of neural cell lines of human (OL) and rat (C6) origins to SARS-associated coronavirus. Both of the neural cell lines showed no apparent cytopathic effects (CPE) by infection but produced virus with infectivity of 10(2-5) per ml, in sharp contrast to the production by infected Vero E6 cells of >10(9) per ml that showed a lytic infection with characteristic rounding CPE. Interestingly, the infection of intestinal cell line CaCo-2 also induced no apparent CPE, with production of the virus at a slightly lower level as that of the Vero E6 cell culture. Notably, the cellular receptor for the virus, angiotensin-converting enzyme 2 was expressed at similar levels on Vero E6 and CaCo-2 cells, but at undetectable levels on OL and C6 cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><name sortKey="Li, Gui Mei" sort="Li, Gui Mei" uniqKey="Li G" first="Gui-Mei" last="Li">Gui-Mei Li</name><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name></noCountry><country name="Japon"><noRegion><name sortKey="Yamashita, Makiko" sort="Yamashita, Makiko" uniqKey="Yamashita M" first="Makiko" last="Yamashita">Makiko Yamashita</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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